Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Increasing the efficiency of CRISPR-Cas9-VQR precise genome editing in rice

298

Typ / Jahr

Journal Article / 2018

Autoren

Hu, Xixun; Meng, Xiangbing; Liu, Qing; Li, Jiayang; Wang, Kejian

Abstract

Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) is a revolutionary technology that enables efficient genomic modification in many organisms. Currently, the wide use of Streptococcus pyogenes Cas9 (SpCas9) primarily recognizes sites harbouring a canonical NGG protospacer adjacent motif (PAM). The newly developed VQR (D1135V/R1335Q/T1337R) variant of Cas9 has been shown to cleave sites containing NGA PAM in rice, which greatly expanded the range of genome editing. However, the low editing efficiency of the VQR variant remains, which limits its wide application in genome editing. In this study, by modifying the single guide RNA (sgRNA) structure and strong endogenous promoters, we significantly increased the editing efficiency of the VQR variant. The modified CRISPR-Cas9-VQR system provides a robust toolbox for multiplex genome editing at sites containing noncanonical NGA PAM.

Keywords

CRISPR-Cas9; efficiency; genome editing; rice.; VQR

Periodical

Plant Biotechnol J (Plant Biotechnology Journal)

Periodical Number

Page range

292–297

Volume

DOI

10.1111/pbi.12771

View Publication

Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
703	Li, Jiayang; Wang, Kejian China	Oryza sativa	CRISPR/Cas9 MOC3	No information SDN1	Basic research Basic research
704	Li, Jiayang; Wang, Kejian China	Oryza sativa	CRISPR/Cas9 GW2	Increase in seed size and thousand grain weight SDN1	Basic research Basic research