What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Pea early-browning virus -mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis

Typ / Jahr

Journal Article / 2018


Ali, Zahir; Eid, Ayman; Ali, Shakila; Mahfouz, Magdy M.


The clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated (Cas9) system has enabled efficient genome engineering in diverse plant species. However, delivery of genome engineering reagents, such as the single guide RNA (sgRNA), into plant cells remains challenging. Here, we report the engineering of Tobacco rattle virus (TRV) and Pea early browning virus (PEBV) to deliver one or multiple sgRNAs into Nicotiana benthamiana and Arabidopsis thaliana (Col-0) plants that overexpress a nuclear localization signal containing Cas9. Our data showed that TRV and PEBV can deliver sgRNAs into inoculated and systemic leaves, and this resulted in mutagenesis of the targeted genomic loci. Moreover, in N. benthamiana, PEBV-based sgRNA delivery resulted in more targeted mutations than TRV-based delivery. Our data indicate that TRV and PEBV can facilitate plant genome engineering and can be used to produce targeted mutations for functional analysis and other biotechnological applications across diverse plant species. Key message: Delivery of genome engineering reagents into plant cells is challenging and inefficient and this limit the applications of this technology in many plant species. RNA viruses such as TRV and PEBV provide an efficient tool to systemically deliver sgRNAs for targeted genome modification.

Arabidopsis; CRISPR/Cas9; Nicotiana benthamiana; Pea early browning virus; Targeted mutagenesis; Tobacco rattle virus; Virus-mediated genome editing; Virus-mediated genome engineering
Virus Research
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
666 Mahfouz, Magdy M.
Saudi Arabia
Arabidopsis thaliana CRISPR/Cas9
Lack trichomes (easily visible phenotypes)
Basic research
Basic research
667 Mahfouz, Magdy M.
Saudi Arabia
Arabidopsis thaliana CRISPR/Cas9
Transparent testa (easily visible phenotypes)
Basic research
Basic research
668 Mahfouz, Magdy M.
Saudi Arabia
Nicotiana benthamiana CRISPR/Cas9
Albino phenotype
Basic research
Basic research