Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Engineering herbicide-resistant maize using chimeric RNA/DNA oligonucleotides

271

Typ / Jahr

Journal Article / 2000

Autoren

Zhu, T.; Mettenburg, K.; Peterson, D. J.; Tagliani, L.; Baszczynski, C. L.

Abstract

Maize plants resistant to imidazolinone herbicides were engineered through targeted modification of endogenous genes using chimeric RNA/DNA oligonucleotides. A precise single-point mutation was introduced into genes encoding acetohydroxyacid synthase (AHAS), at a position known to confer imidazolinone resistance. Phenotypically normal plants from the converted events (C0) were regenerated from resistant calli and grown to maturity. Herbicide leaf painting confirmed the resistance phenotype in C0 plants and demonstrated the anticipated segregation pattern in C1 progeny. DNA cloning and sequencing of the targeted region in resistant calli and derived C0 and C1 plants confirmed the expected mutation. These results demonstrate that oligonucleotide-mediated gene manipulation can be applied to crop improvement. This approach does not involve genomic integration of transgenes. Since the new trait is obtained through modifying a gene within its normal chromosomal context, position effects, transgene silencing, or other concerns that arise as part of developing transgenic events are avoided.

Keywords

Acetohydroxyacid synthase Chimeraplasty; acetolactate synthase; article; Cloning, Molecular; crop; DNA; DNA modification; DNA sequence; DNA, Plant; Drug Resistance; Fungi; Gene modification; gene segregation; Gene silencing; gene targeting; Genes, Plant; genetic engineering; Genetic inheritance; genetic manipulation; herbicide; herbicide resistance; Herbicides; Imidazoles; imidazoline; insecticide resistance; maize; Meiosis; molecular cloning; Mutagenesis, Site-Directed; Myxogastria; Oligodeoxyribonucleotides; oligonucleotide; Oligoribonucleotides; onhuman; plant growth; plant leaf; point mutation; position effect; priority journal; RNA; Selection (Genetics); Sequence Analysis, DNA; Transgene; Zea mays

Periodical

Nature biotechnology

Periodical Number

Page range

555–558

Volume

DOI

10.1038/75435

View Publication

Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
656	Baszczynski, Chris L USA	Zea mays	Oligonucleotide-directed mutagenesis AHAS	Herbicide tolerance PM	Market-oriented Herbicide tolerance