Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9


Typ / Jahr

Journal Article / 2016

Autoren

Zhu, Jinjie; Song, Ning; Sun, Silong; Yang, Weilong; Zhao, Haiming; Song, Weibin; Lai, Jinsheng

Abstract

CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) is an adaptive immune system in bacteria and archaea to defend against invasion from foreign DNA fragments. Recently, it has been developed as a powerful targeted genome editing tool for a wide variety of species. However, its application in maize has only been tested with transiently expressed somatic cells or with a limited number of stable transgenic T0 plants. The exact efficiency and specificity of the CRISPR/Cas system in the highly complex maize genome has not been documented yet. Here we report an extensive study of the well-studied type II CRISPR-Cas9 system for targeted genome editing in maize, with the codon-optimized Cas9 protein and the short non-coding guide RNA generated through a functional maize U6 snRNA promoter. Targeted gene mutagenesis was detected for 90 loci by maize protoplast assay, with an average cleavage efficiency of 10.67%. Stable knockout transformants for maize phytoene synthase gene (PSY1) were obtained. Mutations occurred in germ cells can be stably inherited to the next generation. Moreover, no off-target effect was detected at the computationally predicted putative off-target loci. No significant difference between the transcriptomes of the Cas9 expressed and non-expressed lines was detected. Our results confirmed that the CRISPR-Cas9 could be successfully applied as a robust targeted genome editing system in maize.

Keywords
CRISPR-Associated Proteins/metabolism; CRISPR-Cas Systems; CRISPR-Cas9; Endodeoxyribonucleases/metabolism; gene editing; Genome, Plant; heritability; Inheritance Patterns; maize; mutagenesis; Mutation; Plants, Genetically Modified/genetics; Targeted mutagenesis; Transcription, Genetic; Zea mays/genetics
Periodical
Journal of genetics and genomics = Yi chuan xue bao
Periodical Number
1
Page range
25–36
Volume
43
DOI
10.1016/j.jgg.2015.10.006T

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
655 Jinsheng Lai
China
Zea mays CRISPR/Cas9
PSY1
Albino phenotype
SDN1
Basic research
Basic research