Repositorium
Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
260
Journal Article / 2016
Zhang, Yi; Liang, Zhen; Zong, Yuan; Wang, Yanpeng; Liu, Jinxing; Chen, Kunling; Qiu, Jin-Long; Gao, Caixia
Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing CRISPR/Cas9 introduced as DNA or RNA. This transient expression-based genome-editing system is highly efficient and specific for producing transgene-free and homozygous wheat mutants in the T0 generation. We demonstrate our protocol to edit genes in hexaploid bread wheat and tetraploid durum wheat, and show that we are able to generate mutants with no detectable transgenes. Our methods may be applicable to other plant species, thus offering the potential to accelerate basic and applied plant genome-engineering research.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
625 |
Gao, Caixia China |
Triticum aestivum |
CRISPR/Cas9 GASR7 |
grain length and weight SDN1 |
Basic research Basic research |
626 |
Gao, Caixia China |
Triticum aestivum |
CRISPR/Cas9 GW2 |
negative regulator of kernal width and weight SDN1 |
Basic research Basic research |
627 |
Gao, Caixia China |
Triticum aestivum |
CRISPR/Cas9 DEP1 |
Increasing the yield of rice grain SDN1 |
Basic research Basic research |
628 |
Gao, Caixia China |
Triticum aestivum |
CRISPR/Cas9 LOX2 |
seed storability SDN1 |
Basic research Basic research |
629 |
Gao, Caixia China |
Triticum aestivum |
CRISPR/Cas9 NAC2 |
regulate shoot branching SDN1 |
Basic research Basic research |
630 |
Gao, Caixia China |
Triticum aestivum |
CRISPR/Cas9 PIN1 |
root emergence and tillering SDN1 |
Basic research Basic research |
631 |
Gao, Caixia China |
Triticum turgidum L. var. Durum |
CRISPR/Cas9 GASR7 |
grain length and weight SDN1 |
Basic research Basic research |