Repositorium
TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice
258
Journal Article / 2016
Zhang, Hui; Gou, Feng; Zhang, Jinshan; Liu, Wenshan; Li, Qianqian; Mao, Yanfei; Botella, José Ramón; Zhu, Jian-Kang
CRISPR/Cas9 and TALEN are currently the two systems of choice for genome editing. We have studied the efficiency of the TALEN system in rice as well as the nature and inheritability of TALEN-induced mutations and found important features of this technology. The N287C230 TALEN backbone resulted in low mutation rates (0-6.6%), but truncations in its C-terminal domain dramatically increased efficiency to 25%. In most transgenic T0 plants, TALEN produced a single prevalent mutation accompanied by a variety of low-frequency mutations. For each independent T0 plant, the prevalent mutation was present in most tissues within a single tiller as well as in all tillers examined, suggesting that TALEN-induced mutations occurred very early in the development of the shoot apical meristem. Multigenerational analysis showed that TALEN-induced mutations were stably transmitted to the T1 and T2 populations in a normal Mendelian fashion. In our study, the vast majority of TALEN-induced mutations (~81%) affected multiple bases and ~70% of them were deletions. Our results contrast with published reports for the CRISPR/Cas9 system in rice, in which the predominant mutations affected single bases and deletions accounted for only 3.3% of the overall mutations.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
608 |
Zhu, Jian-kang China, USA |
Oryza sativa |
TALENs CSA |
displays male sterility under short day conditions and male fertility under long day conditions (photoperiod-controlled male sterility ) SDN1 |
Basic research Basic research |
609 |
Zhu, Jian-kang China, USA |
Oryza sativa |
TALENs DERF1 |
drought tolerant SDN1 |
Basic research Basic research |
610 |
Zhu, Jian-kang China, USA |
Oryza sativa |
TALENs GN1a |
enhanced grain yield/ grain number per panicle SDN1 |
Basic research Basic research |
611 |
Zhu, Jian-kang China, USA |
Oryza sativa |
TALENs MST7 |
cold sensitive SDN1 |
Basic research Basic research |
612 |
Zhu, Jian-kang China, USA |
Oryza sativa |
TALENs MST8 |
cold sensitive SDN1 |
Basic research Basic research |
613 |
Zhu, Jian-kang China, USA |
Oryza sativa |
TALENs PMS3 |
Photo-period sensitive male sterile SDN1 |
Basic research Basic research |
614 |
Zhu, Jian-kang China, USA |
Oryza sativa |
TALENs TAD1 |
Dwarf and increased tillers SDN1 |
Basic research Basic research |