Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

MISSA 2.0: An updated synthetic biology toolbox for assembly of orthogonal CRISPR/Cas systems


Typ / Jahr

Journal Article / 2017

Autoren

Zhang, Hai-Yan; Wang, Xing-Hui; Dong, Li; Wang, Zhi-Ping; Liu, Bing; Lv, Jie; Xing, Hui-Li; Han, Chun-Yan; Wang, Xue-Chen; Chen, Qi-Jun

Abstract

Efficient generation of plants carrying mutations in multiple genes remains a challenge. Using two or more orthogonal CRISPR/Cas systems can generate plants with multi-gene mutations, but assembly of these systems requires a robust, high-capacity toolkit. Here, we describe MISSA 2.0 (multiple-round in vivo site-specific assembly 2.0), an extensively updated toolkit for assembly of two or more CRISPR/ Cas systems. We developed a novel suicide donor vector system based on plasmid RK2, which has much higher cloning capacity than the original, plasmid R6K-based system. We validated the utility of MISSA 2.0 by assembling multiple DNA fragments into the E. coli chromosome, and by creating transgenic Arabidopsis thaliana that constitutively or inducibly overexpress multiple genes. We then demonstrated that the higher cloning capacity of the RK2-derived MISSA 2.0 donor vectors facilitated the assembly of two orthogonal CRISPR/Cas systems including SpCas9 and SaCas9, and thus facilitated the creation of transgenic lines harboring these systems. We anticipate that MISSA 2.0 will enable substantial advancements in multiplex genome editing based on two or more orthogonal CRISPR/Cas9 systems, as well as in plant synthetic biology.

Keywords
Periodical
Scientific reports
Periodical Number
Page range
41993
Volume
7
DOI
10.1038/srep41993

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
603 Chen, Qi-Jun
China
Arabidopsis thaliana CRISPR/Cas9
ABI1
ABA and glucose insensitivity
SDN1
Basic research
Basic research
604 Chen, Qi-Jun
China
Arabidopsis thaliana CRISPR/Cas9
ABI2
curled leafes
SDN1
Basic research
Basic research
605 Chen, Qi-Jun
China
Arabidopsis thaliana CRISPR/Cas9
HAB1
curled leafes
SDN1
Basic research
Basic research
606 Chen, Qi-Jun
China
Arabidopsis thaliana CRISPR/Cas9
TRY
curled leafes
SDN1
Basic research
Basic research
607 Chen, Qi-Jun
China
Arabidopsis thaliana CRISPR/Cas9
CPC
curled leafes
SDN1
Basic research
Basic research