What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Targeted mutagenesis in the progeny of maize transgenic plants

Typ / Jahr

Journal Article / 2009


Yang, Meizhu; Djukanovic, Vesna; Stagg, Jessica; Lenderts, Brian; Bidney, Dennis; Falco, S. Carl; Lyznik, L. Alexander


We have demonstrated that targeted mutagenesis can be accomplished in maize plants by excision, activation, and subsequent elimination of an endonuclease in the progeny of genetic crosses. The yeast FLP/FRT site-specific recombination system was used to excise and transiently activate the previously integrated yeast I-SceI homing endonuclease in maize zygotes and/or developing embryos. An artificial I-SceI recognition sequence integrated into genomic DNA was analyzed for mutations to indicate the I-SceI endonuclease activity. Targeted mutagenesis of the I-SceI site occurred in about 1% of analyzed F1 plants. Short deletions centered on the I-SceI-produced double-strand break were the predominant genetic lesions observed in the F1 plants. The I-SceI expression cassette was not detected in the mutant F1 plants and their progeny. However, the original mutations were faithfully transmitted to the next generation indicating that the mutations occurred early during the F1 plant development. The procedure offers simultaneous production of double-strand breaks and delivery of DNA template combined with a large number of progeny plants for future gene targeting experiments.

Base Sequence; Crosses, Genetic; Deoxyribonucleases, Type II Site-Specific/genetics/metabolism; DNA Breaks, Double-Stranded; DNA Probes/genetics; DNA, Bacterial/genetics; DNA, Plant/genetics; DNA, Recombinant/genetics; gene expression; gene targeting; Genes, Fungal; Genetic Vectors; Molecular Sequence Data; mutagenesis; Plants, Genetically Modified; polymerase chain reaction; Saccharomyces cerevisiae Proteins/genetics/metabolism; Saccharomyces cerevisiae/enzymology/genetics; Zea mays/embryology/genetics/metabolism
Plant molecular biology
Periodical Number
Page range


ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
Type of Alteration
Progress in Research
Key Topic
590 Lyznik, Alexander
Zea mays Meganucleases
artificial I-SceI target site near FLP
No information
Basic research
Basic research