High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System

Journal Article / 2015

Yan, Liuhua; Wei, Shaowei; Wu, Yaorong; Hu, Ruolan; Li, Hongju; Yang, Weicai; Xie, Qi

Targeted genome-editing tools are very important and efficient for plant functional genomics research, especially when proper mutants are not available. CRISPR/Cas9-based genome editing is a recently developed powerful technology derived from the bacterial clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) defense system, whereby CRISPR loci were transcribed into non-coding RNAs, which can form a complex with Cas proteins to mediate the cleavage of complementary invading DNA. Since 2012, CRISPR/Cas9based genome editing technology has been successfully applied in human cell lines, zebrafish, and mouse (Cong et al., 2013; Hsu et al., 2014), and used to modify multiple target genes in plants over the past 2 years (Feng et al., 2013; Li et al., 2013; Hyun et al., 2015; Ma et al., 2015; Mao et al., 2013; Xie et al., 2014).