Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice


Typ / Jahr

Journal Article / 2014

Autoren

Xu, Rongfang; Li, Hao; Qin, Ruiying; Wang, Lu; Li, Li; Wei, Pengcheng; Yang, Jianbo

Abstract

BACKGROUND The type II clustered, regularly interspaced, short palindromic repeat (CRISPR)/ CRISPR-associated protein 9 (Cas9) system is a novel molecular tool for site-specific genome modification. The CRISPR-Cas9 system was recently introduced into plants by transient or stable transformation. FINDINGS Here, we report gene targeting in rice via the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system. Three 20-nt CRISPR RNAs were designed to pair with diverse sites followed by the protospacer adjacent motif (PAM) of the rice herbicide resistance gene BEL. After integrating the single-guide RNA (sgRNA) and Cas9 cassette in a single binary vector, transgenic rice plants harboring sgRNA:Cas9 were generated by A. tumefaciens-mediated stable transformation. By analyzing the targeting site on the genome of corresponding transgenic plants, the mutations were determined. The mutagenesis efficiency was varied from ~2% to ~16%. Furthermore, phenotypic analysis revealed that the biallelic mutated transgenic plant was sensitive to bentazon. CONCLUSIONS Our results indicate that the agricultural trait could be purposely modified by sgRNA:Cas9-induced gene targeting. CRISPR-Cas9 system could be exploited as a powerful tool for trait improvements in crop breeding.

Keywords
Agrobacterium; CRISPR/Cas9; gene targeting; genome editing; rice; Stable transformation
Periodical
Rice (New York, N.Y.)
Periodical Number
1
Page range
5
Volume
7
DOI
10.1186/s12284-014-0005-6

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
576 Wei, Peng-Chang; Yang, Jian-Bo
China
Oryza sativa CRISPR/Cas9
BEL
herbicide tolerance
SDN1
Basic research
Basic research