Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Generation of inheritable and "transgene clean" targeted genome-modified rice in later generations using the CRISPR/Cas9 system


Typ / Jahr

Journal Article / 2015

Autoren

Xu, Rong-Fang; Li, Hao; Qin, Rui-Ying; Li, Juan; Qiu, Chun-Hong; Yang, Ya-Chun; Ma, Hui; Li, Li; Wei, Peng-Cheng; Yang, Jian-Bo

Abstract

The CRISPR/Cas9 system is becoming an important genome editing tool for crop breeding. Although it has been demonstrated that target mutations can be transmitted to the next generation, their inheritance pattern has not yet been fully elucidated. Here, we describe the CRISPR/Cas9-mediated genome editing of four different rice genes with the help of online target-design tools. Highfrequency mutagenesis and a large percentage of putative biallelic mutations were observed in T0 generations. Nonetheless, our results also indicate that the progeny genotypes of biallelic T0 lines are frequently difficult to predict and that the transmission of mutations largely does not conform to classical genetic laws, which suggests that the mutations in T0 transgenic rice are mainly somatic mutations. Next, we followed the inheritance pattern of T1 plants. Regardless of the presence of the CRISPR/Cas9 transgene, the mutations in T1 lines were stably transmitted to later generations, indicating a standard germline transmission pattern. Off-target effects were also evaluated, and our results indicate that with careful target selection, off-target mutations are rare in CRISPR/ Cas9-mediated rice gene editing. Taken together, our results indicate the promising production of inheritable and “transgene clean” targeted genome-modified rice in the T1 generation using the CRISPR/Cas9 system.

Keywords
CRISPR-Cas Systems; gene targeting; Genes, Plant; Genome, Plant; genomic instability; Inheritance Patterns; mutagenesis; Mutation; Oryza/genetics; Plants, Genetically Modified; RNA editing; Transgenes
Periodical
Scientific reports
Periodical Number
Page range
11491–11500
Volume
5
DOI
10.1038/srep11491

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
572 Wei, Peng-Chang; Yang, Jian-Bo
China
Oryza sativa CRISPR/Cas9
AOX1a
No information
SDN1
Basic research
Basic research
573 Wei, Peng-Chang; Yang, Jian-Bo
China
Oryza sativa CRISPR/Cas9
AOX1b
No information
SDN1
Basic research
Basic research
574 Wei, Peng-Chang; Yang, Jian-Bo
China
Oryza sativa CRISPR/Cas9
AOX1c
No information
SDN1
Basic research
Basic research
575 Wei, Peng-Chang; Yang, Jian-Bo
China
Oryza sativa CRISPR/Cas9
BEL
herbicide tolerance
SDN1
Basic research
Basic research