Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

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239

Typ / Jahr

Journal Article / 2015

Autoren

Woo, Je Wook; Kim, Jungeun; Kwon, Soon Il; Corvalán, Claudia; Cho, Seung Woo; Kim, Hyeran; Kim, Sang-Gyu; Kim, Sang-Tae; Choe, Sunghwa; Kim, Jin-Soo

Abstract

Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation.

Keywords
Arabidopsis/genetics/physiology; Base Sequence; CRISPR-Cas Systems/genetics; Gene Knockout Techniques; Genetic Engineering/methods; Genome, Plant/genetics; Molecular Sequence Data; Oryza/genetics/physiology; Plant Proteins/genetics/metabolism; Plants, Genetically Modified/genetics/physiology; Protoplasts; Ribonucleoproteins/genetics/metabolism; Tobacco/genetics/physiology
Periodical
Nature biotechnology
Periodical Number
11
Page range
1162–1164
Volume
33
DOI
10.1038/nbt.3389
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Techniques

ID Corresponding Author
Country		 Plant Species GE Technique
Sequence Identifier		 Trait
Type of Alteration		 Progress in Research
Key Topic
557 Choe, Sunghwa; Kim, Jin-Soo
South Korea 		Arabidopsis thaliana CRISPR/Cas9
BRI1 		Dwarf phenotype
SDN1 		Basic research
Basic research
558 Choe, Sunghwa; Kim, Jin-Soo
South Korea 		Arabidopsis thaliana CRISPR/Cas9
AOC 		encodes a key enzyme for jasmonic acid biosynthesis
SDN1 		Basic research
Basic research
559 Choe, Sunghwa; Kim, Jin-Soo
South Korea 		Oryza sativa CRISPR/Cas9
DWD1 		No information
SDN1 		Basic research
Basic research
560 Choe, Sunghwa; Kim, Jin-Soo
South Korea 		Oryza sativa CRISPR/Cas9
P450 		No information
SDN1 		Basic research
Basic research
561 Choe, Sunghwa; Kim, Jin-Soo
South Korea 		Lactuca sativa CRISPR/Cas9
BIN2 		No information
SDN1 		Basic research
Basic research
562 Choe, Sunghwa; Kim, Jin-Soo
South Korea 		Nicotiana attenuata CRISPR/Cas9
PHYB 		No information
SDN1 		Basic research
Basic research