Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants


Typ / Jahr

Journal Article / 2013

Autoren

Wendt, Toni; Holm, Preben Bach; Starker, Colby G.; Christian, Michelle; Voytas, Daniel F.; Brinch-Pedersen, Henrik; Holme, Inger Bæksted

Abstract

Transcription activator-like effector nucleases (TALENs) enable targeted mutagenesis in a variety of organisms. The primary advantage of TALENs over other sequence-specific nucleases, namely zinc finger nucleases and meganucleases, lies in their ease of assembly, reliability of function, and their broad targeting range. Here we report the assembly of several TALENs for a specific genomic locus in barley. The cleavage activity of individual TALENs was first tested in vivo using a yeast-based, single-strand annealing assay. The most efficient TALEN was then selected for barley transformation. Analysis of the resulting transformants showed that TALEN-induced double strand breaks led to the introduction of short deletions at the target site. Additional analysis revealed that each barley transformant contained a range of different mutations, indicating that mutations occurred independently in different cells.

Keywords
Base Sequence; Endonucleases/metabolism; Genome, Plant; Hordeum/genetics; Molecular Sequence Data; Mutation; Sequence Homology, Nucleic Acid; Transcription Factors/metabolism; Transformation, Genetic
Periodical
Plant molecular biology
Periodical Number
3
Page range
279–285
Volume
83
DOI
10.1007/s11103-013-0078-4

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
556 Wendt, Toni
Denmark
Hordeum vulgare TALENs
Paphy_a
Altered phytase expression and activity
SDN1
Basic research
Basic research