Repositorium
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew
232
Journal Article / 2014
Wang, Yanpeng; Cheng, Xi; Shan, Qiwei; Zhang, Yi; Liu, Jinxing; Gao, Caixia; Qiu, Jin-Long
Sequence-specific nucleases have been applied to engineer targeted modifications in polyploid genomes, but simultaneous modification of multiple homoeoalleles has not been reported. Here we use transcription activator-like effector nuclease (TALEN) and clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 (refs. 4,5) technologies in hexaploid bread wheat to introduce targeted mutations in the three homoeoalleles that encode MILDEW-RESISTANCE LOCUS (MLO) proteins. Genetic redundancy has prevented evaluation of whether mutation of all three MLO alleles in bread wheat might confer resistance to powdery mildew, a trait not found in natural populations. We show that TALEN-induced mutation of all three TaMLO homoeologs in the same plant confers heritable broad-spectrum resistance to powdery mildew. We further use CRISPR-Cas9 technology to generate transgenic wheat plants that carry mutations in the TaMLO-A1 allele. We also demonstrate the feasibility of engineering targeted DNA insertion in bread wheat through nonhomologous end joining of the double-strand breaks caused by TALENs. Our findings provide a methodological framework to improve polyploid crops.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
545 |
Gao, Caixia; Qiu, Jin-Long China |
Triticum aestivum |
TALENs MLO |
Powdery mildew resistance SDN1 |
Market-oriented Biotic stress tolerance |
546 |
Gao, Caixia; Qiu, Jin-Long China |
Triticum aestivum |
CRISPR/Cas9 MLO |
Fungus stress tolrerance - tolerant to powdrey mildew SDN1 |
Basic research Basic research |