Repositorium
Journal Article / 2017
Wang, Mugui; Mao, Yanfei; Lu, Yuming; Tao, Xiaoping; Zhu, Jian-Kang
The class 2/type II clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpress multiple single guide RNAs (sgRNAs) to achieve multiplex gene editing in plant cells. One is to construct the multiple guide RNA expression cassettes into separate plasmids when direct gene delivery methods are adopted, such as biolistic bombardment and PEG-mediated protoplast transfection (Shan et al., 2013). The other is to assemble the multiple sgRNAs into a single vector when the Agrobacteria-mediated gene transformation method is used. These multiple sgRNAs can be driven by separate promoters (Ma et al., 2015; Zhang et al., 2016) or expressed as a single transcript for further processing by plant endogenous ribonucleases (Xie et al., 2015). Although these two strategies were reported to be efficient for introducing targeted gene modifications in plant genomes, the construction of CRISPR/ Cas9 vectors was complicated and laborious.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
534 |
Zhu, Jian-kang China, USA |
Oryza sativa |
CRISPR/Cpf1 EPSPS |
herbicide tolerance SDN1 |
Basic research Basic research |
535 |
Zhu, Jian-kang China, USA |
Oryza sativa |
CRISPR/Cpf1 BEL |
herbicide tolerance SDN1 |
Basic research Basic research |
536 |
Zhu, Jian-kang China, USA |
Oryza sativa |
CRISPR/Cpf1 PDS |
Albino and dwarf phenotype SDN1 |
Basic research Basic research |
537 |
Zhu, Jian-kang China, USA |
Oryza sativa |
CRISPR/Cpf1 RLKs |
No information SDN1 |
Basic research Basic research |