What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

Typ / Jahr

Journal Article / 2016


Vazquez-Vilar, Marta; Bernabé-Orts, Joan Miquel; Fernandez-Del-Carmen, Asun; Ziarsolo, Pello; Blanca, Jose; Granell, Antonio; Orzaez, Diego


Background: The efficiency, versatility and multiplexing capacity of RNA‑guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the construction of transcriptional gene circuits, many of which depend on the technical ability to compose and transfer complex synthetic instructions into the plant cell. The engineering principles of standardization and modularity applied to DNA cloning are impacting plant genetic engineering, by increasing multigene assembly efficiency and by fostering the exchange of well‑defined physical DNA parts with precise functional information. Results: Here we describe the adaptation of the RNA‑guided Cas9 system to GoldenBraid (GB), a modular DNA construction framework being increasingly used in Plant Synthetic Biology. In this work, the genetic elements required for CRISPRs‑based editing and transcriptional regulation were adapted to GB, and a workflow for gRNAs construction was designed and optimized. New software tools specific for CRISPRs assembly were created and incorporated to the public GB resources site. Conclusions: The functionality and the efficiency of gRNA–Cas9 GB tools were demonstrated in Nicotiana benthamiana using transient expression assays both for gene targeted mutations and for transcriptional regulation. The availability of gRNA–Cas9 GB toolbox will facilitate the application of CRISPR/Cas9 technology to plant genome engineering. Keywords: Plant gene editing, Plant gene activation, Plant gene repression, CRISPR/Cas9, gRNAs, Multigenic assemblies, GoldenBraid, Luciferase/renilla assay

Plant gene editing Plant gene activation Plant gene repression CRISPR/Cas9 gRNAs Multigenic assemblies GoldenBraid Luciferase/renilla assay
Plant methods
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ID Corresponding Author
Plant Species GE Technique
Sequence Identifier
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Key Topic
510 Orzaez, Diego
Nicotiana benthamiana CRISPR/Cas9
No information
Basic research
Basic research