Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

High-frequency modification of plant genes using engineered zinc-finger nucleases


Typ / Jahr

Journal Article / 2009

Autoren

Townsend, Jeffrey A.; Wright, David A.; Winfrey, Ronnie J.; Fu, Fengli; Maeder, Morgan L.; Joung, J. Keith; Voytas, Daniel F.

Abstract

An efficient method for making directed DNA sequence modifications to plant genes (gene targeting) is at present lacking, thereby frustrating efforts to dissect plant gene function and engineer crop plants that better meet the world’s burgeoning need for food, fibre and fuel. Zinc-finger nucleases (ZFNs)—enzymes engineered to create DNA double-strand breaks at specific loci—are potent stimulators of gene targeting1,2 ; for example, they can be used to precisely modify engineered reporter genes in plants3,4 . Here we demonstrate high-frequency ZFN-stimulated gene targeting at endogenous plant genes, namely the tobacco acetolactate synthase genes (ALS SuRA and SuRB), for which specific mutations are known to confer resistance to imidazolinone and sulphonylurea herbicides5 . Herbicide-resistance mutations were introduced into SuR loci by ZFN-mediated gene targeting at frequencies exceeding 2% of transformed cells for mutations as far as 1.3 kilobases from the ZFN cleavage site. More than 40% of recombinant plants had modifications in multiple SuR alleles. The observed high frequency ofgene targeting indicates that it is nowpossible to efficientlymake targeted sequence changes in endogenous plant genes.

Keywords
Acetolactate Synthase/genetics; Alleles; Amino Acid Sequence; Base Sequence; Deoxyribonucleases/chemistry/genetics/metabolism; Food, Genetically Modified; Gene Targeting/methods; Genes, Plant/genetics; Herbicide Resistance/genetics; Herbicides/pharmacology; Molecular Sequence Data; Plants, Genetically Modified; Protein Engineering; Recombination, Genetic/genetics; Tobacco/drug effects/enzymology/genetics; Transformation, Genetic; Zinc Fingers
Periodical
Nature
Periodical Number
7245
Page range
442–445
Volume
459
DOI
10.1038/nature07845

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
499 Voytas, Daniel F.
USA
Tobacco Zinc-finger nucleases
SuRA
herbicide tolerance
SDN1
Basic research
Basic research
500 Voytas, Daniel F.
USA
Tobacco Zinc-finger nucleases
SuRB
herbicide tolerance
SDN1
Basic research
Basic research
501 Voytas, Daniel F.
USA
Tobacco Zinc-finger nucleases
SuRA
herbicide tolerance
SDN2
Basic research
Basic research
502 Voytas, Daniel F.
USA
Tobacco Zinc-finger nucleases
SuRB
herbicide tolerance
SDN2
Basic research
Basic research