Repositorium
A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants
210
Journal Article / 2017
Tang, Xu; Lowder, Levi G.; Zhang, Tao; Malzahn, Aimee A.; Zheng, Xuelian; Voytas, Daniel F.; Zhong, Zhaohui; Chen, Yiyi; Ren, Qiurong; Li, Qian; Kirkland, Elida R.; Zhang, Yong; Qi, Yiping
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cpf1 has emerged as an effective genome editing tool in animals. Here we compare the activity of Cpf1 from Acidaminococcus sp. BV3L6 (As) and Lachnospiraceae bacterium ND2006 (Lb) in plants, using a dual RNA polymerase II promoter expression system. LbCpf1 generated biallelic mutations at nearly 100% efficiency at four independent sites in rice T0 transgenic plants. Moreover, we repurposed AsCpf1 and LbCpf1 for efficient transcriptional repression in Arabidopsis, and demonstrated a more than tenfold reduction in miR159b transcription. Our data suggest promising applications of CRISPR-Cpf1 for editing plant genomes and modulating the plant transcriptome.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
483 |
Zhang, Yong; Qi, Yiping China, USA |
Oryza sativa |
CRISPR/Cpf1 DEP1 |
Grain number/ grain yield/ plant hight/ spukelet number SDN1 |
Basic research Basic research |
484 |
Zhang, Yong; Qi, Yiping China, USA |
Oryza sativa |
CRISPR/Cpf1 PDS |
Albino and dwarf phenotype SDN1 |
Basic research Basic research |
485 |
Zhang, Yong; Qi, Yiping China, USA |
Oryza sativa |
CRISPR/Cpf1 ROC5 |
Curly leaf phenotype SDN1 |
Basic research Basic research |