Repositorium
Journal Article / 2016
Svitashev, Sergei; Schwartz, Christine; Lenderts, Brian; Young, Joshua K.; Mark Cigan, A.
Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on delivering double-strand break reagents in the form of DNA vectors. Here we report biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. Using this method of delivery, we also demonstrate DNA- and selectable marker-free gene mutagenesis in maize and recovery of plants with mutated alleles at high frequencies. These results open new opportunities to accelerate breeding practices in a wide variety of crop species.
Techniques
ID | Corresponding Author Country |
Plant Species | GE Technique Sequence Identifier |
Trait Type of Alteration |
Progress in Research Key Topic |
---|---|---|---|---|---|
467 |
Svitashev, Sergei USA |
Zea mays |
CRISPR/Cas9 MS26 |
Male sterility SDN1 |
Market-oriented Agronomic value |
468 |
Svitashev, Sergei USA |
Zea mays |
CRISPR/Cas9 MS26 |
Male sterility SDN1 |
Market-oriented Agronomic value |
469 |
Svitashev, Sergei USA |
Zea mays |
CRISPR/Cas9 MS45 |
Male sterility SDN1 |
Market-oriented Agronomic value |
470 |
Svitashev, Sergei USA |
Zea mays |
CRISPR/Cas9 MS45 |
Male sterility SDN1 |
Market-oriented Agronomic value |
471 |
Svitashev, Sergei USA |
Zea mays |
CRISPR/Cas9 ALS2 |
Herbicide tolerance SDN1 |
Market-oriented Herbicide tolerance |
472 |
Svitashev, Sergei USA |
Zea mays |
CRISPR/Cas9 ALS2 |
Herbicide tolerance SDN1 |
Market-oriented Herbicide tolerance |
473 |
Svitashev, Sergei USA |
Zea mays |
CRISPR/Cas9 ALS2 |
Herbicide tolerance SDN3 |
Market-oriented Herbicide tolerance |
474 |
Svitashev, Sergei USA |
Zea mays |
CRISPR/Cas9 LIG |
Disrupted formation of leaf cuticular wax (liguleless) SDN1 |
Basic research Basic research |
475 |
Svitashev, Sergei USA |
Zea mays |
CRISPR/Cas9 LIG |
Disrupted formation of leaf cuticular wax (liguleless) SDN1 |
Basic research Basic research |