Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes


Typ / Jahr

Journal Article / 2016

Autoren

Svitashev, Sergei; Schwartz, Christine; Lenderts, Brian; Young, Joshua K.; Mark Cigan, A.

Abstract

Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on delivering double-strand break reagents in the form of DNA vectors. Here we report biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. Using this method of delivery, we also demonstrate DNA- and selectable marker-free gene mutagenesis in maize and recovery of plants with mutated alleles at high frequencies. These results open new opportunities to accelerate breeding practices in a wide variety of crop species.

Keywords
Periodical
Nature communications
Periodical Number
Page range
1–7
Volume
7
DOI
10.1038/ncomms13274

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
467 Svitashev, Sergei
USA
Zea mays CRISPR/Cas9
MS26
Male sterility
SDN1
Market-oriented
Agronomic value
468 Svitashev, Sergei
USA
Zea mays CRISPR/Cas9
MS26
Male sterility
SDN1
Market-oriented
Agronomic value
469 Svitashev, Sergei
USA
Zea mays CRISPR/Cas9
MS45
Male sterility
SDN1
Market-oriented
Agronomic value
470 Svitashev, Sergei
USA
Zea mays CRISPR/Cas9
MS45
Male sterility
SDN1
Market-oriented
Agronomic value
471 Svitashev, Sergei
USA
Zea mays CRISPR/Cas9
ALS2
Herbicide tolerance
SDN1
Market-oriented
Herbicide tolerance
472 Svitashev, Sergei
USA
Zea mays CRISPR/Cas9
ALS2
Herbicide tolerance
SDN1
Market-oriented
Herbicide tolerance
473 Svitashev, Sergei
USA
Zea mays CRISPR/Cas9
ALS2
Herbicide tolerance
SDN3
Market-oriented
Herbicide tolerance
474 Svitashev, Sergei
USA
Zea mays CRISPR/Cas9
LIG
Disrupted formation of leaf cuticular wax (liguleless)
SDN1
Basic research
Basic research
475 Svitashev, Sergei
USA
Zea mays CRISPR/Cas9
LIG
Disrupted formation of leaf cuticular wax (liguleless)
SDN1
Basic research
Basic research