Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Creation of fragrant rice by targeted knockout of the OsBADH2 gene using TALEN technology


Typ / Jahr

Journal Article / 2015

Autoren

Shan, Qiwei; Zhang, Yi; Chen, Kunling; Zhang, Kang; Gao, Caixia

Abstract

Fragrant rice is favoured worldwide because of its agreeable scent. The presence of a defective badh2 allele encoding betaine aldehyde dehydrogenase (BADH2) results in the synthesis of 2-acetyl-1-pyrroline (2AP), which is a major fragrance compound. Here, transcription activator-like effector nucleases (TALENs) were engineered to target and disrupt the OsBADH2 gene. Six heterozygous mutants (30%) were recovered from 20 transgenic hygromycin-resistant lines. Sanger sequencing confirmed that these lines had various indel mutations at the TALEN target site. All six transmitted the BADH2 mutations to the T1 generation; and four T1 mutant lines tested also efficiently transmitted the mutations to the T2 generation. Mutant plants carrying only the desired DNA sequence change but not the TALEN transgene were obtained by segregation in the T1 and T2 generations. The 2AP content of rice grains of the T1 lines with homozygous mutations increased from 0 to 0.35-0.75 mg/kg, which was similar to the content of a positive control variety harbouring the badh2-E7 mutation. We also simultaneously introduced three different pairs of TALENs targeting three separate rice genes into rice cells by bombardment and obtained lines with mutations in one, two and all three genes. These results indicate that targeted mutagenesis using TALENs is a useful approach to creating important agronomic traits.

Keywords
Base Sequence; Betaine-Aldehyde Dehydrogenase/genetics; DNA, Plant; Gene Knockdown Techniques; Genes, Plant; Molecular Sequence Data; Mutation; Oryza/enzymology/genetics; Sequence Homology, Nucleic Acid
Periodical
Plant biotechnology journal
Periodical Number
6
Page range
791–800
Volume
13
DOI
10.1111/pbi.12312

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
436 Gao, Caixia
China
Oryza sativa TALENs
BADH2
Fragrant genotype
SDN1
Market-oriented
Product quality
437 Gao, Caixia
China
Oryza sativa TALENs
CKX2
Increasing the yield of rice grain
SDN1
Basic research
Basic research
438 Gao, Caixia
China
Oryza sativa TALENs
DEP1
Increasing the yield of rice grain
SDN1
Basic research
Basic research