Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)

181

Typ / Jahr

Journal Article / 2013

Autoren

Sander, Jeffry D.; Dahlborg, Elizabeth J.; Goodwin, Mathew J.; Cade, Lindsay; Zhang, Feng; Cifuentes, Daniel; Curtin, Shaun J.; Blackburn, Jessica S.; Thibodeau-Beganny, Stacey; Qi, Yiping; Pierick, Christopher J.; Hoffman, Ellen; Maeder, Morgan L.; Khayter, Cyd; Reyon, Deepak; Dobbs, Drena; Langenau, David M.; Stupar, Robert M.; Giraldez, Antonio J.; Voytas, Daniel F.; Peterson, Randall T.; Yeh, Jing-Ruey J.; Joung, J. Keith

Abstract

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe context-dependent assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA-generated ZFNs, we rapidly altered 20 genes in Danio rerio, Arabidopsis thaliana and Glycine max. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multigene pathways or genome-wide alterations.

Keywords

*Protein Engineering; Animals Arabidopsisgenetics; DNA-Binding Proteinsgeneticsmetabolism; Endonucleases*genetics*metabolism; genome; Soybeansgenetics; Zebrafishgenetics; Zinc Fingersgenetics*physiology

Periodical

Nat Meth (Nature Methods)

Periodical Number

Page range

67–71

Volume

DOI

10.1038/nmeth.1542

View Publication

Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
394	Joung, J.Keith USA	Arabidopsis thaliana	Zinc-finger nucleases GA3OX2	No information SDN1	Basic research Basic research
395	Joung, J.Keith USA	Arabidopsis thaliana	Zinc-finger nucleases MAPKKK18	No information SDN1	Basic research Basic research
396	Joung, J.Keith USA	Arabidopsis thaliana	Zinc-finger nucleases MKK9	No information SDN1	Basic research Basic research
397	Joung, J.Keith USA	Arabidopsis thaliana	Zinc-finger nucleases MPK8	No information SDN1	Basic research Basic research
398	Joung, J.Keith USA	Arabidopsis thaliana	Zinc-finger nucleases MPK15	No information SDN1	Basic research Basic research
399	Joung, J.Keith USA	Arabidopsis thaliana	Zinc-finger nucleases MPK11	No information SDN1	Basic research Basic research
400	Joung, J.Keith USA	Glycine Max	Zinc-finger nucleases DclLa	strong morphological phenotype, characterized by reduced seed size and aborted seedling development SDN1	Basic research Basic research
401	Joung, J.Keith USA	Glycine Max	Zinc-finger nucleases DclLb	strong morphological phenotype, characterized by reduced seed size and aborted seedling development SDN1	Basic research Basic research