Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit


Typ / Jahr

Journal Article / 2017

Autoren

Ordon, Jana; Gantner, Johannes; Kemna, Jan; Schwalgun, Lennart; Reschke, Maik; Streubel, Jana; Boch, Jens; Stuttmann, Johannes

Abstract

Genome editing facilitated by Cas9-based RNA-guided nucleases (RGNs) is becoming an increasingly important and popular technique for reverse genetics in both model and non-model species. So far, RGNs were mainly applied for the induction of point mutations, and one major challenge consists in the detection of genome-edited individuals from a mutagenized population. Also, point mutations are not appropriate for functional dissection of non-coding DNA. Here, the multiplexing capacity of a newly developed genome editing toolkit was exploited for the induction of inheritable chromosomal deletions at six different loci in Nicotiana benthamiana and Arabidopsis. In both species, the preferential formation of small deletions was observed, suggesting reduced efficiency with increasing deletion size. Importantly, small deletions (<100 bp) were detected at high frequencies in N. benthamiana T0 and Arabidopsis T2 populations. Thus, targeting of small deletions by paired nucleases represents a simple approach for the generation of mutant alleles segregating as size polymorphisms in subsequent generations. Phenotypically selected deletions of up to 120 kb occurred at low frequencies in Arabidopsis, suggesting larger population sizes for the discovery of valuable alleles from addressing gene clusters or non-coding DNA for deletion by programmable nucleases.

Keywords
Arabidopsis thaliana; chromosomal deletion; CRISPR/Cas; Eds1; Nicotiana benthamiana; plant immunity; technical advance.
Periodical
The Plant journal : for cell and molecular biology
Periodical Number
1
Page range
155–168
Volume
89
DOI
10.1111/tpj.13319

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
343 Stuttmann, Johannes
Germany
Arabidopsis thaliana CRISPR/Cas9
DM2h
No information
SDN1
Basic research
Basic research
344 Stuttmann, Johannes
Germany
Arabidopsis thaliana CRISPR/Cas9
DMC2c
No information
SDN1
Basic research
Basic research
345 Stuttmann, Johannes
Germany
Arabidopsis thaliana CRISPR/Cas9
GUS
No information
SDN1
Basic research
Basic research
346 Stuttmann, Johannes
Germany
Nicotiana benthamiana CRISPR/Cas9
EDS1a/b
No information
SDN1
Basic research
Basic research
347 Stuttmann, Johannes
Germany
Nicotiana benthamiana CRISPR/Cas9
GUS
GUS expression
SDN1
Basic research
Basic research
348 Stuttmann, Johannes
Germany
Nicotiana benthamiana CRISPR/Cas9
PNbPAD4
No information
SDN1
Basic research
Basic research