Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice

160

Typ / Jahr

Journal Article / 2004

Autoren

Okuzaki, A.; Toriyama, K.

Abstract

Site-specific mutagenesis in a rice genome was obtained by introducing chimeric RNA/DNA oligonucleotides (COs) by means of particle bombardment. Three COs were designed to target the independent codons for Pro-171, Trp-548 and Ser-627 of the endogenous rice acetolactate synthase (ALS) gene so it would confer resistance to ALS-inhibiting herbicides. Sequencing of the ALS gene of herbicide-resistant plants demonstrated that the ALS sequence was modified in a site-specific fashion. The efficiency of gene conversion mediated by COs was estimated to be 1x10(-4). These results demonstrate that CO-directed gene targeting is feasible in rice.

Keywords

Acetolactate Synthase/genetics; Base Sequence; Biolistics; Chimera/genetics; DNA, Plant/genetics; DNA, Recombinant/genetics; Drug Resistance/genetics; gene conversion; Gene Targeting/methods; Genome, Plant; Herbicides/pharmacology; Mutagenesis, Site-Directed; Oligonucleotides/genetics; Oryza/drug effects/enzymology/genetics; Plants, Genetically Modified; RNA, Plant/genetics; RNA/genetics

Periodical

Plant cell reports

Periodical Number

Page range

509–512

Volume

DOI

10.1007/s00299-003-0698-2

View Publication

Techniques

ID	Corresponding Author Country	Plant Species	GE Technique Sequence Identifier	Trait Type of Alteration	Progress in Research Key Topic
342	Toriyama, K. Japan	Oryza sativa	Oligonucleotide-directed mutagenesis ALS	herbicide tolerance PM	Market-oriented Herbicide tolerance