Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Efficient and Heritable Targeted Mutagenesis in Mosses Using the CRISPR/Cas9 System


Typ / Jahr

Journal Article / 2016

Autoren

Nomura, Toshihisa; Sakurai, Tetsuya; Osakabe, Yuriko; Osakabe, Keishi; Sakakibara, Hitoshi

Abstract

Targeted genome modification by RNA-guided nucleases derived from the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system has seen rapid development in many organisms, including several plant species. In the present study, we succeeded in introducing the CRISPR/Cas9 system into the non-model organism Scopelophila cataractae, a moss that exhibits heavy metal tolerance, and the model organism Physcomitrella patens Utilizing the process by which moss plants regenerate from protoplasts, we conducted targeted mutagenesis by expression of single-chain guide RNA (sgRNA) and Cas9 in protoplasts. Using this method, the acquisition rate of strains exhibiting phenotypic changes associated with the target genes was approximately 45-69%, and strains with phenotypic changes exhibited various insertion and deletion mutations. In addition, we report that our method is capable of multiplex targeted mutagenesis (two independent genes) and also permits the efficient introduction of large deletions (∼3 kbp). These results demonstrate that the CRISPR/Cas9 system can be used to accelerate investigations of bryology and land plant evolution.

Keywords
Bryophyta/genetics; Clustered Regularly Interspaced Short Palindromic Repeats/genetics; CRISPR-Cas Systems; Endonucleases/genetics; gene editing; Mutagenesis, Site-Directed; Mutation; Protoplasts; RNA, Guide/genetics; RNA, Plant/genetics
Periodical
Plant & cell physiology
Periodical Number
12
Page range
2600–2610
Volume
57
DOI
10.1093/pcp/pcw173

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
338 Sakakibara, Hitoshi. Toshihisa Nomura
Japan
Physcomitrella patens CRISPR/Cas9
FtsZ2-1
responsible for chloroplast division
SDN1
Basic research
Basic research
339 Sakakibara, Hitoshi. Toshihisa Nomura
Japan
Physcomitrella patens CRISPR/Cas9
TON
small gametophores with short and thickened leaves
SDN1
Basic research
Basic research
340 Sakakibara, Hitoshi. Toshihisa Nomura
Japan
Scopelophila cataractae CRISPR/Cas9
FtsZ2-1
responsible for chloroplast division
SDN1
Basic research
Basic research