Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Two novel NAC transcription factors regulate gene expression and flowering time by associating with the histone demethylase JMJ14


Typ / Jahr

Journal Article / 2015

Autoren

Ning, Yong-Qiang; Ma, Ze-Yang; Huang, Huan-Wei; Mo, Huixian; Zhao, Ting-ting; Li, Lin; Cai, Tao; Chen, She; Ma, Ligeng; He, Xin-Jian

Abstract

The histone demethylase JMJ14 catalyzes histone demethylation at lysine 4 of histone 3 and is involved in transcriptional repression and flowering time control in Arabidopsis. Here, we report that JMJ14 is physically associated with two previously uncharacterized NAC transcription factors, NAC050 and NAC052. The NAC050/052-RNAi plants and the CRISPR-CAS9-mediated nac050/052 double mutant plants show an early flowering phenotype, which is similar to the phenotype of jmj14, suggesting a functional association between JMJ14 and NAC050/052. RNA-seq data indicated that hundreds of common target genes are co-regulated by JMJ14 and NAC50/052. Our ChIP analysis demonstrated that JMJ14 and NAC050 directly bind to co-upregulated genes shared in jmj14 and NAC050/052-RNAi, thereby facilitating H3K4 demethylation and transcriptional repression. The NAC050/052 recognition DNA cis-element was identified by an electrophoretic mobility shift assay at the promoters of its target genes. Together, our study identifies two novel NAC transcription repressors and demonstrates that they are involved in transcriptional repression and flowering time control by associating with the histone demethylase JMJ14.

Keywords
Chromatography, Affinity; Chromatography, Gel; Clustered Regularly Interspaced Short Palindromic Repeats; Flowers/physiology; Gene Expression Regulation/physiology; Histone Demethylases/metabolism; mass spectrometry; Transcription Factors/physiology; Two-Hybrid System Techniques
Periodical
Nucleic acids research
Periodical Number
3
Page range
1469–1484
Volume
43
DOI
10.1093/nar/gku1382

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
334 He, Xin-Jian
China
Arabidopsis thaliana CRISPR/Cas9
NAC
Early flowering genotype
SDN1
Basic research
Basic research