Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Disruption of Phytoene Desaturase Gene using Transient Expression of Cas9


Typ / Jahr

Journal Article / 2016

Autoren

Mubarik, M. Salman; Khan, Sultan H.; Ahmad, Aftab; Khan, Zulqurnain; Sajjad, Muhammad; Khan, Iqrar A.

Abstract

Engineered nucleases have emerged as a powerful tool for site specific gene manipulation in plants. Based on Clustered Regularly Interspersed Short Palindromic Repeats/CRISPR associated (CRISPR/Cas) system, engineered Cas9:gRNA complex can be used to cleave specific DNA sequences in the genome. In the present study, Nicotiana benthamiana Phytoene Desaturase (NbPDS) gene was targeted by CRISPR/Cas9 system. The plant codon optimized (pcoCas9) along with guided RNA (gRNA) was cloned in plant expression vector pGreen0029, to target NbPDS gene. The NbPDS gene was disrupted transiently by agroinfiltration of pcoCas9-gRNA complex. Visible albino spots were observed on agro-infiltrated leaves of N. benthamiana plants after 7 days of infiltration. The observed albino spots were analyzed through PCR amplification of gRNAtarget, fluorescent microscopy and chlorophyll contents measurements. Our results support the notion that CRISPR/Cas9 system is a swift, robust and useful tool for targeted gene disruption, deletion and editing. © 2016 Friends Science Publishers

Keywords
CRISPR/Cas9 system; NbPDS gene; Site specific genome editing; Transient expression
Periodical
IJAB (International Journal of Agriculture and Biology)
Periodical Number
Page range
990–996
Volume
DOI
10.17957/IJAB/15.0199

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
328 Khan, Sultan Habibullah
Pakistan
Nicotiana benthamiana CRISPR/Cas9
PDS
Albino and dwarf phenotype
SDN1
Basic research
Basic research