Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Non-transgenic Plant Genome Editing Using Purified Sequence-Specific Nucleases


Typ / Jahr

Journal Article / 2015

Autoren

Luo, Song; Li, Jin; Stoddard, Thomas J.; Baltes, Nicholas J.; Demorest, Zachary L.; Clasen, Benjamin M.; Coffman, Andrew; Retterath, Adam; Mathis, Luc; Voytas, Daniel F.; Zhang, Feng

Abstract

Sequence-specific nucleases, including zinc-finger nucleases, meganucleases, TAL effector nucleases (TALENs), and CRISPR/ Cas systems, have been used to introduce targeted mutations in a wide range of plant species (Voytas, 2013; Baltes and Voytas, 2015). However, delivery of these nucleases using traditional transformation methods (e.g., particle bombardment, Agrobacterium or protoplast transformation) may result in undesired genetic alterations due to random insertion of nucleaseencodingDNAinto thehost genome. Randomintegration ofDNA is a particular concern for trait improvement and gene function studies, because it may lead to unintended gene inactivation or it may alter expression of host genes. Furthermore, for crop varieties created using biotechnology, the presence of foreign DNA is a trigger for regulation by many governmental agencies (Voytas and Gao, 2014). Therefore, methods to modify plant genomes that do not require DNA delivery would have value in both commercial and academic settings. Here we demonstrate nontransgenic plant genome engineering by introducing sequencespecific nucleases as purified protein. This approach enabled targetedmutagenesis ofendogenoussequenceswithinplant cells, while avoiding integration of foreign DNA into the genome.

Keywords
Base Sequence; Endonucleases/metabolism; Genome, Plant; Molecular Sequence Data; Mutagenesis/genetics; Plants, Genetically Modified; RNA Editing/genetics; Tobacco/genetics; Trans-Activators/metabolism
Periodical
Molecular plant
Periodical Number
9
Page range
1425–1427
Volume
8
DOI
10.1016/j.molp.2015.05.012

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
289 Luo, Song
USA
Nicotiana tabacum TALENs
ALS2
herbicide tolerance
SDN1
Basic research
Basic research
290 Luo, Song
USA
Nicotiana tabacum Meganucleases
YFP
Yellow fluorescent protein
SDN1
Basic research
Basic research