Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Precise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System


Typ / Jahr

Journal Article / 2017

Autoren

Lu, Yuming; Zhu, Jian-Kang

Abstract

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated Cas9) has been widely used in genome editing in a variety of organisms, including rice (Cong et al., 2013; Feng et al., 2013). The majority of the editing events reported in plants typically result in an abundance of random insertions and deletions (Indels) at the target locus through the error-prone non-homologous end joining pathway (NHEJ) (Feng et al., 2013; Mao et al., 2013; Shan et al., 2013), making genome editing an extremely valuable tool for plant functional genomics research. Most known agriculturally important traits, however, are conferred by point mutations (Huang et al., 2010; Hu et al., 2015). Development of a technique that enables precise and efficient base replacement in the target locus, rather than stochastic disruption of the gene, will greatly facilitate precision plant molecular breeding. Homology-directed repair (HDR) is a potential approach to achieve base replacement by providing a homologous DNA template during genome editing. However, HDR is extremely inefficient due to competition by NHEJ, the dominant pathway to repair double-strand breaks (DSBs) in plants, and Indels are generally much more abundant outcomes, making HDR a rather ineffective method to achieve base replacement in plants (Li et al., 2013; Mao et al., 2013).

Keywords
Periodical
Molecular plant
Periodical Number
3
Page range
523–525
Volume
10
DOI
10.1016/j.molp.2016.11.013

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
287 Zhu, Jian-Kang
China, USA
Oryza sativa Base-editing
SLR
reduced plant height
BE
Market-oriented
Agronomic value
288 Zhu, Jian-Kang
China, USA
Oryza sativa Base-editing
NRT1.1B
increased nitrogene use efficience
BE
Basic research
Basic research