Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation


Typ / Jahr

Journal Article / 2015

Autoren

Lowder, Levi G.; Zhang, Dengwei; Baltes, Nicholas J.; Paul, Joseph W.; Tang, Xu; Zheng, Xuelian; Voytas, Daniel F.; Hsieh, Tzung-Fu; Zhang, Yong; Qi, Yiping

Abstract

The relative ease, speed, and biological scope of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated Protein9 (Cas9)-based reagents for genomic manipulations are revolutionizing virtually all areas of molecular biosciences, including functional genomics, genetics, applied biomedical research, and agricultural biotechnology. In plant systems, however, a number of hurdles currently exist that limit this technology from reaching its full potential. For example, significant plant molecular biology expertise and effort is still required to generate functional expression constructs that allow simultaneous editing, and especially transcriptional regulation, of multiple different genomic loci or multiplexing, which is a significant advantage of CRISPR/Cas9 versus other genome-editing systems. To streamline and facilitate rapid and wide-scale use of CRISPR/Cas9-based technologies for plant research, we developed and implemented a comprehensive molecular toolbox for multifaceted CRISPR/Cas9 applications in plants. This toolbox provides researchers with a protocol and reagents to quickly and efficiently assemble functional CRISPR/Cas9 transfer DNA constructs for monocots and dicots using Golden Gate and Gateway cloning methods. It comes with a full suite of capabilities, including multiplexed gene editing and transcriptional activation or repression of plant endogenous genes. We report the functionality and effectiveness of this toolbox in model plants such as tobacco (Nicotiana benthamiana), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa), demonstrating its utility for basic and applied plant research.

Keywords
Periodical
Plant physiology
Periodical Number
2
Page range
971–985
Volume
169
DOI
10.1104/pp.15.00636

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
280 Zhang, Yong; Qi, Yiping
China, USA
Oryza sativa CRISPR/Cas9
ROC5
Curly leaf phenotype
SDN1
Basic research
Basic research
281 Zhang, Yong; Qi, Yiping
China, USA
Oryza sativa CRISPR/Cas9
YSA
Albino leaf phenotype
SDN1
Basic research
Basic research
282 Zhang, Yong; Qi, Yiping
China, USA
Nicotiana benthamiana CRISPR/Cas9
BAK1
important immune receptor or coreceptor genes
SDN1
Basic research
Basic research
283 Zhang, Yong; Qi, Yiping
China, USA
Nicotiana benthamiana CRISPR/Cas9
FLS2
important immune receptor or coreceptor genes
SDN1
Basic research
Basic research