Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana


Typ / Jahr

Journal Article / 2015

Autoren

Liu, Wenshan; Zhu, Xiaohong; Lei, Mingguang; Xia, Qingyou; Botella, Jose Ramon; Zhu, Jian-Kang; Mao, Yanfei

Abstract

The newly developed CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system has emerged as an efficient tool for genome-editing, providing an alternative to classical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR/Cas facilitates targeted gene editing through RNA-guided DNA cleavage followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. Here we describe a detailed procedure for our previously developed and highly efficient CRISPR/Cas9 method that allows the generation of heritable-targeted gene mutations and corrections in Arabidopsis. This protocol describes the strategies and steps for the selection of targets, design of single-guide RNA (sgRNA), vector construction and analysis of transgenic lines. We also offer a method to target two loci simultaneously using vectors containing two different sgRNAs. The principles described in this protocol can be applied to other plant species to generate stably inherited DNA modifications.

Keywords
CRISPR/Cas9; Targeted gene editing; Genome engineering; Arabidopsis thaliana;
Periodical
Science Bulletin
Periodical Number
15
Page range
1332–1347
Volume
60
DOI
10.1007/s11434-015-0848-2

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
263 Xia, Qingyou, Mao, Yanfei
China
Arabidopsis thaliana CRISPR/Cas9
No information
No information
SDN1
Basic research
Basic research