Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Receptor-Like Kinase RUPO Interacts with Potassium Transporters to Regulate Pollen Tube Growth and Integrity in Rice


Typ / Jahr

Journal Article / 2016

Autoren

Liu, Lingtong; Zheng, Canhui; Kuang, Baijan; Wei, Liqin; Yan, Longfeng; Wang, Tai

Abstract

During sexual reproduction of flowering plants, the pollen tube grows fast and over a long distance within the pistil to deliver two sperms for double fertilization. Growing plant cells need to communicate constantly with external stimuli as well as monitor changes in surface tension of the cell wall and plasma membrane to coordinate these signals and internal growth machinery; however, the underlying mechanisms remain largely unknown. Here we show that the rice member of plant-specific receptor-like kinase CrRLK1Ls subfamily, Ruptured Pollen tube (RUPO), is specifically expressed in rice pollen. RUPO localizes to the apical plasma membrane and vesicle of pollen tubes and is required for male gamete transmission. K+ levels were greater in pollen of homozygous CRISPR-knockout lines than wild-type plants, and pollen tubes burst shortly after germination. We reveal the interaction of RUPO with high-affinity potassium transporters. Phosphorylation of RUPO established and dephosphorylation abolished the interaction. These results have revealed the receptor-like kinase as a regulator of high-affinity potassium transporters via phosphorylation-dependent interaction, and demonstrated a novel receptor-like kinase signaling pathway that mediates K+ homeostasis required for pollen tube growth and integrity.

Keywords
Angiosperms/genetics/growth & development; Arabidopsis Proteins/genetics; Arabidopsis/genetics; Flowers/genetics/growth & development; Gene Expression Regulation, Plant; Germination/genetics; Homeostasis; Membrane Proteins/biosynthesis/genetics; Oryza/genetics/growth & development; Phosphorylation; Plant Proteins/genetics; Pollen Tube/genetics/growth & development; Pollen/genetics/growth & development; Pollination/genetics; Potassium-Hydrogen Antiporters/biosynthesis/genetics; Protein Kinases/genetics; Receptor Protein-Tyrosine Kinases/genetics
Periodical
PLoS genetics
Periodical Number
7
Page range
Volume
12
DOI
10.1371/journal.pgen.1006085

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
262 Wang, Tai
China
Oryza sativa CRISPR/Cas9
RUPO
Regulation of pollen tube growth and integrity
SDN1
Market-oriented
Agronomic value