Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system


Typ / Jahr

Journal Article / 2014

Autoren

Liang, Zhen; Zhang, Kang; Chen, Kunling; Gao, Caixia

Abstract

Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated (Cas) systems have emerged as powerful tools for genome editing in a variety of species. Here, we report, for the first time, targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system. We designed five TALENs targeting 4 genes, namely ZmPDS, ZmIPK1A, ZmIPK, ZmMRP4, and obtained targeting efficiencies of up to 23.1% in protoplasts, and about 13.3% to 39.1% of the transgenic plants were somatic mutations. Also, we constructed two gRNAs targeting the ZmIPK gene in maize protoplasts, at frequencies of 16.4% and 19.1%, respectively. In addition, the CRISPR/Cas system induced targeted mutations in Z. mays protoplasts with efficiencies (13.1%) similar to those obtained with TALENs (9.1%). Our results show that both TALENs and the CRISPR/Cas system can be used for genome modification in maize.

Keywords
Base Sequence; Clustered Regularly Interspaced Short Palindromic Repeats/genetics; CRISPR/Cas system; CRISPR-Cas Systems/genetics; DNA Restriction Enzymes/metabolism; Genes, Plant/genetics; Genetic Engineering/methods; Genomics; knock-out; Mutagenesis, Site-Directed; Mutation; Plants, Genetically Modified; TAL-effector nucleases; Zea mays; Zea mays/genetics
Periodical
Journal of genetics and genomics = Yi chuan xue bao
Periodical Number
2
Page range
63–68
Volume
41
DOI
10.1016/j.jgg.2013.12.001

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
257 Gao, Caixia
China
Zea mays CRISPR/Cas9
IPK
Reduction of phytic acid
SDN1
Basic research
Basic research
258 Gao, Caixia
China
Zea mays TALENS
IPK/1A
Reduction of phytic acid
SDN1
Basic research
Basic research
259 Gao, Caixia
China
Zea mays TALENS
MRP4
No information
SDN1
Basic research
Basic research
260 Gao, Caixia
China
Zea mays TALENS
PDS
Albino and dwarf phenotype
SDN1
Basic research
Basic research