Repositorium

What is a repositorium?

The repositorium is a searchable database that provides data on relevant articles from journals, company web pages and web pages of governmental agencies about studies/applications of genome-editing in model plants and agricultural crops in the period January 1996 to May 2018. Search options are article type, technique, plant, traits or free text. The repositorium is based on the systematic map of Dominik Modrzejewski et al., published in the journal environmental evidence. (Download article PDF).

Cas9-Guide RNA Directed Genome Editing in Soybean


Typ / Jahr

Journal Article / 2015

Autoren

Li, Zhongsen; Liu, Zhan-Bin; Xing, Aiqiu; Moon, Bryan P.; Koellhoffer, Jessica P.; Huang, Lingxia; Ward, R. Timothy; Clifton, Elizabeth; Falco, S. Carl; Cigan, A. Mark

Abstract

Recently discovered bacteria and archaea adaptive immune system consisting of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) endonuclease has been explored in targeted genome editing in different species. Streptococcus pyogenes Cas9-guide RNA (gRNA) was successfully applied to generate targeted mutagenesis, gene integration, and gene editing in soybean (Glycine max). Two genomic sites, DD20 and DD43 on chromosome 4, were mutagenized with frequencies of 59% and 76%, respectively. Sequencing randomly selected transgenic events confirmed that the genome modifications were specific to the Cas9-gRNA cleavage sites and consisted of small deletions or insertions. Targeted gene integrations through homology-directed recombination were detected by border-specific polymerase chain reaction analysis for both sites at callus stage, and one DD43 homology-directed recombination event was transmitted to T1 generation. T1 progenies of the integration event segregated according to Mendelian laws and clean homozygous T1 plants with the donor gene precisely inserted at the DD43 target site were obtained. The Cas9-gRNA system was also successfully applied to make a directed P178S mutation of acetolactate synthase1 gene through in planta gene editing.

Keywords
Bacterial Proteins/genetics; DNA End-Joining Repair; Endonucleases/genetics; Genetic Engineering/methods; Genome, Plant; homologous recombination; Mutagenesis, Insertional; Plant Proteins/genetics; Plants, Genetically Modified; RNA editing; RNA, Guide; Soybeans/drug effects/genetics; Sulfonamides/pharmacology; Triazines/pharmacology
Periodical
Plant physiology
Periodical Number
2
Page range
960–970
Volume
169
DOI
10.1104/pp.15.00783

Techniques

ID Corresponding Author
Country
Plant Species GE Technique
Sequence Identifier
Trait
Type of Alteration
Progress in Research
Key Topic
238 Li, Zhongsen
USA
Glycine Max CRISPR/Cas9
ALS
herbicide tolerance
SDN2
Market-oriented
Herbicide tolerance
239 Li, Zhongsen
USA
Glycine Max CRISPR/Cas9
DD20
No information
SDN1
Basic research
Basic research
240 Li, Zhongsen
USA
Glycine Max CRISPR/Cas9
DD43
No information
SDN1
Basic research
Basic research
241 Li, Zhongsen
USA
Glycine Max CRISPR/Cas9
HPT into DD43
hygromycin resistance gene
SDN3
Basic research
Basic research